Genetic Engineering -

• Can we change our genes?
• Do we want to change our genes?

Tools of the gene engineer

• Splicing Enzymes
  • Restriction Enzymes - Flash model - Modification
  • Ligation Enzyme
• Cloning vectors
  • Plasmids
  • Cosmids
  • BACs and YACs
• DNA for Cloning
  • Genomic
  • cDNA
• Building libraries
• Screening libraries
• Catalogues of genes

In vitro Propagation of DNA

• PCR - Polymerase Chain Reaction
  • Flash 4 rendition
  • details of PCR
  • Cycle sequencing
• Public Libraries
• NCBIhttp://www.ncbi.nlm.nih.gov/
• Sanger Center Ensembl.org
• TIGR http://www.tigr.org/