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Mixed Co-Polymer Experiments

In these experiments a mixture of two nucleotides were used to produce the basic RNA polymer produced. Digfferent ratios of the bases/nucleotides were used in the experemental procedure used earlier. Predicted results and interpretation of a mixed copolymer experiment where a ratio of 1A to 5 C was used.

Possibl e
Compo sitions

Concentratio ns

Probability of Occurance of any Triplet

Possible Triplets

Final %

3C

(5/6)(5/6)(5/6 )

128/216=57.9 %

CCC

57.9%

1A:2C

(1/6)(5/6)(5/6 )

25/216=11.6%

ACC CAC CCA

 3 (11.6%) = 34.8%

2A:1C

(5/6)(1/6)(1/6 )

5/216 = 2.3%

AAC ACA CAA

3 (2.3%) = 6.9%

3A

(1/6)(1/6)(1/6 )

1/216 = 0.4%

AAA

0.4%

Amino acid incorporation as a results of mixed Homopolymer experiment with a ratio of 1 part A : 5 parts C

Amino Acids in Protein

% in Protein

Probable Base Composition and Assignments

Proline

69

CCC, 2C:1A

Histidine

14

2C:1 A, 1C:2A

Threonine

12

2C:1A

Aspargine

2

1C:2A

Glutamine

2

1C:2A

Lysine

1

AAA

 
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This web site is provided for instruction in Botany and Zoology 342 or Genetics 342

Text: Genetics: A conceptural ApproachBenjamin A Pierce

This is our friend Oliver the parrotlet, a true parrot, the smallest in the world.


 

 by Kenneth G. Wilson,
Professor of Botany
Miami University
wilsonkg@muohio.edu