MBI 414/514 - Immunology Principles
MBI 415/515 - Immunology Principles and Practice

Syllabus

Lecture Outline - Fall 2012

August 20
Introduction and Course Overview
Basic Concepts in Imumunology (Chapter 1)
August 22
Basic Concepts in Imumunology (Chapter 1)
Innate Immunity: First Lines of Defense (Chapter 2)
August 24
Innate Immunity: Complement System (Chapter 2)
August 27
Innate Immunity: Complement System (Chapter 2)
August 29
Induced Responses of Innate Immunity: Pattern Recognion (Chapter 3)
August 31
Induced Responses of Innate Immunity: Pattern Recognion (Chapter 3)
September 3
Labor Day - No Class
September 5
Induced Responses of Innate Immunity: Responses to Infection (Chapter 3)
September 7
Induced Responses of Innate Immunity: Responses to Infection (Chapter 3)
Article Report 1 Due
September 10
Examination 1 (Covers Chapters 1-3)
September 12
Antigen Recognition by B Cell and T Cell Receptors: Antibody Structure and Function (Chapter 4)
September 14
Antigen Recognition by B Cell and T Cell Receptors: Antibody Structure and Function (Chapter 4)
September 17
Antigen Recognition by B Cell and T Cell Receptors: T Cell Receptor Structure and Function (Chapter 4)
September 19
Antigen Recognition by B Cell and T Cell Receptors: T Cell Receptor Structure and Function (Chapter 4)
September 21
Generation of Lymphocyte Antigen Receptors (Chapter 5)
September 24
Generation of Lymphocyte Antigen Receptors (Chapter 5)
Article Report 2 - Article Submission Due
September 26
Antigen Presentation to T Lymphocytes (Chapter 6)
September 28
Antigen Presentation to T Lymphocytes (Chapter 6)
Article Report 2 - Report Submission Due
October 1
Examination 2 (Covers Chapters 4-6)
October 3
Signaling Through Immune System Receptors (Chapter 7)
October 5
Signaling Through Immune System Receptors (Chapter 7)
October 8
Signaling Through Immune System Receptors (Chapter 7)
October 10
Signaling Through Immune System Receptors (Chapter 7)
October 12
MidTerm Break - No Class
October 15
Development and Survival of Lymphocytes (Chapter 8)
Article Report 3 - Article Submission Due
October 17
Development and Survival of Lymphocytes (Chapter 8)
October 19
Development and Survival of Lymphocytes (Chapter 8)
October 22
Development and Survival of Lymphocytes (Chapter 8)
Article Report 3 - Report Submission Due
October 24
Examination 3 (Covers Chapters 7-8)
October 26
T Cell Mediated Immunity (Chapter 9)
Article Report 4 - Article Submission Due
October 29
T Cell Mediated Immunity (Chapter 9)
October 31
Humoral Immune Response (Chapter 10)
November 2
Humoral Immune Response (Chapter 10)
November 5
Dynamics of Adaptive Immunity (Chapter 11)
November 7
Dynamics of Adaptive Immunity (Chapter 11)
November 9
Mucosal Immune Response (Chapter 12)
November 12
Mucosal Immune Response (Chapter 12)
Article Report 4 - Report Submission Due
November 14
Examination 4 (Covers Chapters 9-12)
November 16
Failures of Host Defense Mechanisms (Chapter 13)
Article Report 5 - Article Submission Due
November 19
Failures of Host Defense Mechanisms (Chapter 13)
November 21 & 23
Thanksgiving Break - No Class
November 26
Allergy and Allergic Diseases (Chapter 14)
November 28
Allergy and Allergic Diseases (Chapter 14)
November 30
Autoimmunity and Transplantation (Chapter 15)
December 3
Autoimmunity and Transplantation (Chapter 15)
December 5
Manipulation of the Immune Response (Chapter 16)
December 7
Manipulation of the Immune Response (Chapter 16)
Article Report 5 - Report Submission Due
December 12
Final Examination (12:30 - 2:30 pm; Comprehensive... Covers Chapters 1-16)

Textbook - MBI 414/514 and MBI 415/515


Course Policies - MBI 414/514 and MBI 415/515


Course Objectives - MBI 414/514

Immunology Principles (MBI 414/514) is a 3 credit-hour lecture course that will acquaint you with the molecules, cells and organs of the immune system. You will learn about the structural features of the components of the immune system as well as their functions, but the primary emphasis of this course will be on the mechanisms involved in immune system development and responsiveness. The major experiments that allowed the elucidation of these mechanistic features will be featured to help you understand how immunologists think and work.

Graduate Students (MBI 514) must also actively participate in Immunology Journal Club, which will meet every week during the semester to discuss recently published papers in the professional literature of immunology.


Course Evaluation - MBI 414/514

Your course grade will be determined by your performance on:


Your course grade in MBI 414/514 will be determined using the following scale:

Cheating on any aspect of this course may make you subject to immediate dismissal from the class with a grade of F.


Course Objectives - MBI 415/515

Immunology Principles and Practice (MBI 415/515) is a 4 credit-hour lecture-laboratory course that will acquaint you with experimental laboratory techniques in addition to acquainting you with the molecules, cells and organs of the immune system.

The lecture portion of this course (3 credit hours) will acquaint you with the molecules, cells and organs of the immune system. You will learn about the structural features of the components of the immune system as well as their functions, but the primary emphasis of this course will be on the mechanisms involved in immune system development and responsiveness. The major experiments that allowed the elucidation of these mechanistic features will be featured to help you understand how immunologists think and work.

The laboratory portion of this course (1 credit hour) will provide a selective approach to your learning of certain immunologic techniques and assays. The field of immunology has continued to develop at a rapid pace during the latter years of the 20th century and the early years of the 21st century. Consequently, numerous assays and techniques are available to the professional immunologist. It would not be possible to incorporate all of these in any single course. Therefore, this laboratory course will provide a selective approach to learning only some of the more important immunologic techniques and assays.


Laboratory Exercise Outline - MBI 415/515

August 20
Introduction and Overview
Dilution Techniques
August 27
Agglutination
September 3
Labor Day Week - No Laboratory Sessions
September 10
Quantitative Precipitation
September 17
Ouchterlony Double Diffusion
Radial Immunodiffusion
September 24
Immunoelectrophoresis
Immunoblot Analysis
October 1
Fluorescent Microscopy
Flow Cytometry
Review for Laboratory Examination 1
October 8
Examination 1 (Safety... Flow Cytometry)
Notebook Assessment 1
October 15
Enzyme-Linked Immunosorbent Assay
TLRs and Cytokine Production
October 22
TLRs and Cytokines - IL-12 Assay
October 29
TLRs and Cytokines - TNFa and NO Assays
November 5
Phagocytosis and Opsonization
November 12
Bacterial Clearance
November 19
Antibody Response Analysis
Laboratory Report Due (TLRs and Cytokines)
November 26
Hypersensitivity and Immunopathology
Review for Laboratory Examination 2
Course Evaluation
December 3
Examination 2 (Enzyme-Linked Immunosorbent Assay... Hypersensitivity and Immunopathology)
Notebook Assessment 2

Laboratory Manual - MBI 415/515

Immunology Laboratory Manual. 2012. John R. Stevenson and Joseph M. Carlin, Department of Microbiology, Miami University, Oxford, OH.


Laboratory Instructors - MBI 415/515


Laboratory Attendance - MBI 415/515

You are expected to participate in every laboratory exercise and only one excused absence will be given to students with valid medical excuses or reasons acceptable to their TA. Each additional absence will result in deduction of ten percentage points from your final percentage.


Laboratory Preparation - MBI 415/515

For best performance of the laboratory exercises and best understanding of the course material, it is important to approach the laboratory exercises in a thoughtful, organized way. This includes reading each exercise and writing a half-page of laboratory preparation informationfor each lab exercise on what you will be doing during class before you come to the laboratory. You must turn in this "lab prep info” at the before class begins as evidence of your advanced preparation. Your lab prep info must contain a description of the experimental approach to be used in the exercise together with a description of how the data will be reported. (Note: some days have more than one lab exercise; therefore, you must write more than one lab prep.) Failure to turn in this information on time will result in deduction of five points from your total course points.


Laboratory Quizzes - MBI 415/515

On occasion, your Laboratory Instructor (TA) will give you an unannounced quiz on the material he/she thinks you should understand to best perform the exercise(s) you will be performing in laboratory that day.


Laboratory Notebook - MBI 415/515

Your notebook must be an up-to-date, accurate account of everything you do in this laboratory. It should use the same basic format as your laboratory reports, but is not expected to be as rigorous. This is highly important if you are to get the most out of your laboratory experiences this semester. Your laboratory instructor will assess your notebook twice during the semester to assure that you are developing the record-keeping habits expected of professional microbiologists. Your notebook entries should follow this format:


Laboratory Report - MBI 415/515

You will generate one laboratory report based on the Antibody Response Exercise. It should be well integrated, and follow this format:

In preparing your laboratory reports, use appropriate material from your textbook, laboratory manual, or lecture notes as well as other books and articles. You should not, however, simply "regurgitate" any of this material (especially the procedural details), because the object of this report is for you to integrate the information and present it in a clear and thoughtful manner in your own words. These reports must be generated using a computer-based word processor. The Department of Microbiology has a computer laboratory set up for student use. Ask your laboratory instructors when you can use this facility to complete your report. Please remember that plagiarism will not be tolerated!


Course Evaluation - MBI 415/515

Your lecture grade (75% of your course grade) will be determined by your performance on:


Your laboratory grade (25% of your course grade) will be determined by your performance on:

Plagiarism and other infractions of Miami University rules and regulations will be dealt with as described in Chapter 5: Academic Integrity in the Student Handbook.

Your course grade in MBI 415/515 will be determined using the following scale:

Cheating on any aspect of this course may make you subject to immediate dismissal from the class with a grade of F.


Credit/No-Credit Status

Warning: Nationwide studies have shown that credit/no-credit grades on your academic record may be a negative factor in evaluation of your application for admission or employment by most professional schools (law, medicine, etc.), by many graduate schools, and by some employers and undergraduate schools. Before enrolling for courses on a credit/no-credit basis consider what

The following are only the most problematic of the Credit/No-Credit rules... A full-text version can be accessed in Section 1.2.H of Chapter 2 of Part 1: Undergraduate Academic Regulations of the Student Handbook.

Students should consult with the Chief Departmental Adviser of their department of major with questions pertaining to courses that may be taken on a credit/no-credit basis.


Withdrawal Policy


Syllabus

Laboratory

Instructor

Study Guides

Sample Questions

Cool Immuno Stuff

Study Tips

Immuno FAQs

Bugs'n'Drugs


© 1996-2012 John R. Stevenson. All Rights Reserved

Please
email questions and comments to:
John R. Stevenson, Ph.D.
Associate Professor
Department of Microbiology
Miami University
Oxford, Ohio 45056
USA

This document was last modified on Saturday, 17-Nov-2012 23:37:45 EST