PROCEDURES FOR CAPTURE OF WOOD MICROSCOPY IMAGES

I.  INITIAL SET UP OF INSTRUMENTATION

     A.  Make sure Kohler Illumination is set up and maintained throughout the
     imaging process.

     B.  Attach the RGB CCD to the microscope and the computer.  Make sure the
     cables are plugged into the right sockets in the frame capture board = Sync, Red,
     Green, Blue; from Left to Right, facing the back of the computer.

     C.  Boot the computer for Color Imaging Configuration.  Turn on the Sony
     Monitor. 

     D.  Select New Image1 (Item H) from the Shell.  You will use the THREE
     BUTTON mouse to interface with Image1.

     E.   Plug in and turn on the RGB camera.  Position the Optical Path of the
     microscope for split beam (half to oculars, half to CCD).

     F.  In Image1:

          1.  Set Video Source to Channel 3.

          2.  Set Video Mode to RGB, External Sync, Separate Signal.  You should
          see a black and white image of your specimen at this point.

III.  CAPTURE OF MICROSCOPY IMAGES.

     A.  Execute either the RGB Wood Journal (saves images to e:/wood directory) or
     the RGB Image Journal (you specify the directory that images are saved in).

     B.  Compose your image initially using ocular viewing, finally using image
     displayed on Sony Monitor.

     C.  As per journal directions, click the right mouse button to frame average 256
     frames into the R, G, B frame buffers (three clicks total).  Click the right mouse
     button one more time to encode the RGB pallete to a 256 grey level pallete.  The
     final encoded image is displayed from the Results buffer.

     D.  If satisfied, save the encoded image.