Center for Bioinformatics & Functional Genomics
at Miami University
Quantitative real-time PCR (qRT-PCR), or qPCR, is an important application supported by the CBFG. For these experiments, we use:
- 2 Corbett Rotor-Gene 3000™ systems
- Bio-Rad iCycler
These instruments are calibrated for most of the fluorophores that are commonly used for qPCR experiments.
Experimental design is critical to success in qPCR experiments. The CBFG staff provides training and consultation for all aspects of qPCR ranging from experimental design and instrument operation to data interpretation and validation.